Figure 4
From: Shoot organogenesis and somatic embryogenesis from leaf and root explants of Scaevola sericea
Histological observation of somatic embryogenesis from leaf explants of Scaevola sericea. Leaf explants were cultured on MS medium with 2.5 mg/L BA for 0, 10, 20, 30–40, 50, and 60 days (a, b, c, d, e, f, g, respectively). Bars = 0.5 mm; Marked: mc: mesophyll cell; ec: embryogenic cells; gse: a globular somatic embryo; h: hypocotyl, p: plumule. (a) fresh leaf section; (b) culture for 10 days showing some smaller embryogenic cells; (c) culture for 20 days showing some larger embryogenic cells clumps; d, culture for 30–40 days showing a globular somatic embryo with a hypocotyl attached; e, culture for 50 days showing a heart-shaped somatic embryo and plumule meristem development; f, culture for 60 days showing plumule development.
