Figure 2 | Scientific Reports

Figure 2

From: Autoantibodies from SLE patients induce programmed cell death in murine fibroblast cells through interaction with TNFR1 receptor

Figure 2

Autoantibodies induce necroptosis with the involvement of cell organelles in target cells. (A) Cell death of L929 cells incubated with aAb alone or in the presence of RIP1 and MLKL inhibitors for 20 h. (B) Western blot analysis of cytoplasmic proteins with antibodies against p-RIPK1 and p-MLKL in L929 cells incubated with aAb for different periods of time. Protein bands were quantified by using ImageJ software. The data represent the density of each band normalized to corresponding b-actin band. (C) Cell death of L929 cells incubated with aAb alone or in the presence of EGTA, ionol, Necrox-2 and inhibitors of calpains, cathepsins, STAT3, and PL2.

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