Figure 1 | Scientific Reports

Figure 1

From: Polymerized human hemoglobin facilitated modulation of tumor oxygenation is dependent on tumor oxygenation status and oxygen affinity of the hemoglobin-based oxygen carrier

Figure 1

Biophysical properties of unmodified hHb, 35:1 T-state PolyhHb, and 30:1 R-State PolyhHb. (A) OECs for PolyhHb and hHb. (B) Comparison of the kinetic time course for deoxygenation in the presence of 1.5 mg/mL sodium dithionite for hHb, 30:1 R-state PolyhHb, and T-state PolyhHb. For OECs (A), The shaded region represents the 95% confidence interval for each quaternary state with three runs per sample. For deoxygenation (B), the experimental data shows an average of 10–15 kinetic traces. For deoxygenation, the reactions were monitored at 437.5 nm and 20 °C in 0.1 M pH 7.4 PBS. Symbols represent experimental data, and corresponding lines of the same color represent curve fits. (C) Representative intensity distributions of the hydrodynamic diameter of 30:1 R-state and 35:1 T-state PolyhHb. (D) Normalized SEC intensity distributions of R-state and T-state PolyhHb compared to unmodified hHb. The shaded region represents the 95% confidence interval for the average of the produced PolyhHb. (E) Polymer order distribution for 35:1 T-state and 30:1 R-state PolyhHb. Polymer distribution was calculated on a percent by heme basis via analysis of the 413 nm absorbance wavelength. The corresponding approximate sizes of the polymer orders are shown below each group.

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