Table 1 Efficiency in the establishment of the naturally conceived and vitrified-transferred progenies across three generations (F1, F2, F3).

From: Long-term and transgenerational phenotypic, transcriptional and metabolic effects in rabbit males born following vitrified embryo transfer

Experimental groups

F1 generation

F2 generation

F3 generation

Founding parities

Litter size

Live births

Adult males

F1 parities

Litter size

Live births

Adult males

F2 parities

Litter size

Live births

Adult males

Naturally conceived

14

5.5 ± 0.62

77

35

10

6.1 ± 0.76

61

13

10

6.1 ± 0.89

61

24

Vitrified-transferred

13

5.3 ± 0.64

69

30

10

5.6 ± 0.76

56

9

10

6.4 ± 0.89

64

24

  1. In F1 generation, naturally-conceived animals were generated from 14 pregnant females following natural conception, without embryo manipulation. Instead, vitrified-transferred animals were generated from vitrified-warmed embryos transferred in 13 foster mothers. F2 and F3 generations were produced selecting randomly one mature female and male from each parity of the previous generation. To reduce the inbreeding, mating between animals with common grandparents was avoided. Thereby, 10 F1 and 10 F2 parities generated F2 and F3 population, respectively.