Figure 5 | Scientific Reports

Figure 5

From: Offspring of obese mice display enhanced intake and sensitivity for palatable stimuli, with altered expression of taste signaling elements

Figure 5

Taste bud morphology is similar in offspring of NC (blue) and HFD (red) fed dams. Bars denote mean ± SEM. (A) Image of anterior tongue of NC offspring stained with methylene blue to visualize fungiform papillae. (B) Anterior tongue of HFD offspring. (C) Fungiform density within 1 mm × 1 mm square in NC (n = 18) and HFD (n = 21) offspring. (D) Image of circumvallate taste buds of NC offspring. (E) HFD offspring. (F) quantification of taste buds/circumvallate papilla. (G) H&E stained CV papilla of NC offspring. (H) H&E image of HFD offspring papilla. (I) Taste bud size between treatment groups. (J) Immunofluorescent image of Type II taste cells in NC offspring. (K) Type II cells in HFD offspring. (L) Quantification of Type II cells. (M) Immunofluorescent image of sweet/bitter sensitive taste cells in NC offspring (green) with taste bud boundary highlighted (red). (N) Sweet/umami cells in HFD offspring. (O) Quantification of sweet/umami taste cells. Counting was performed from every 8 section of immunofluorescent stains, stars denote significance, where *p < 0.05; **p < 0.01; ***p < 0.001.

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