Figure 3

Effect of the presence of glycine during formaldehyde treatment on the subsequent enzymatic degradation rate of cytochrome c upon digestion by cathepsin S. Peptide formation was quantified by using LC–MS for samples/conditions CYT-FG0-1, CYT-F128-1 and CYT-FG128-1. Peptide peak areas are corrected for the internal standard peak area. Error bars represent the SD of three LC–MS runs. (A) Kinetics of the cytochrome c peptide GLFG. (B) Kinetics of the cytochrome c peptide MIFA. (C) Kinetics of the cytochrome c peptide EETLM. (D) Kinetics of the cytochrome c peptide QAPGFT.