Table 2 Reaction conditions of protein formaldehyde treatment.

From: Formaldehyde treatment of proteins enhances proteolytic degradation by the endo-lysosomal protease cathepsin S

Reaction condition code

Protein

Formaldehyde concentration (mM)

Glycine concentration (mM)

Incubation time (weeks)

BSA-FG0-6

Bovine serum albumin

0

0

6

BSA-FG2-6

Bovine serum albumin

2

2

6

BSA-FG16-6

Bovine serum albumin

16

16

6

BSA-FG80-6

Bovine serum albumin

80

80

6

BSA-FG128-6

Bovine serum albumin

128

128

6

BLG-FG0-6

ß-lactoglobulin A

0

0

6

BLG-FG2-6

ß-lactoglobulin A

2

2

6

BLG-FG16-6

ß-lactoglobulin A

16

16

6

BLG-FG80-6

ß-lactoglobulin A

80

80

6

BLG-FG128-6

ß-lactoglobulin A

128

128

6

CYT-FG0-6

Cytochrome c

0

0

6

CYT-FG2-6

Cytochrome c

2

2

6

CYT-FG16-6

Cytochrome c

16

16

6

CYT-FG80-6

Cytochrome c

80

80

6

CYT-FG128-6

Cytochrome c

128

128

6

CYT-FG0-1

Cytochrome c

0

0

1

CYT-F128-1

Cytochrome c

128a

0

1

CYT-FG128-1

Cytochrome c

128a

128b

1

  1. aThe reaction was performed with 128 mM CH2O and 128 mM CD2O separately, and the reaction products were mixed 1:1 based on protein concentration afterwards.
  2. bThe reaction was performed with 128 mM glycine and 128 mM glycine-1-13C separately, and the reaction products were mixed 1:1 based on protein concentration afterwards. CD2O and glycine-1-13C were used in the same reaction.
Back to article page