Table 1 Results of whole-genome sequencing of S. pyogenes NCTC 8198T and CCUG 4207T, from the four sequencing runs done with PacBio RSII, Illumina HiSeq 2,500 and Oxford Nanopore MinION platforms.

From: Complete genome sequences of Streptococcus pyogenes type strain reveal 100%-match between PacBio-solo and Illumina-Oxford Nanopore hybrid assemblies

Platform

PacBio RSII

Illumina HiSeq 2500

Oxford Nanopore MinION

Run SRA accession number

ERR550482

ERR550487

SRR8631872

SRR10092043

Total number of reads

163,469

163,274

10,260,942

135,020

Total yield (Mb)

420

694

1,293

1,122

Sequencing depth

219 X

362 X

675 X

586 X

Average read length (bp)

2,570

4,250

126

8,307

Read length N50 (bp)

4,555

9,809

126

14,598

Longest read (bp)

35,267

41,160

126

97,527

Average Phred quality score

2.7

3.8

35

11.5

  1. The total number of reads, total yield (Mb), sequencing depth, average read length (bp), read length N50 (bp), longest read (bp) and average Phred quality score are shown for each sequencing run. The SRA accession number of each run is indicated.
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