Figure 2 | Scientific Reports

Figure 2

From: Therapeutic vaccine-mediated Gag-specific CD8+ T-cell induction under anti-retroviral therapy augments anti-virus efficacy of CD8+ cells in simian immunodeficiency virus-infected macaques

Figure 2

SIV antigen-specific CD8+ T-cell responses after SIVmac239 infection. (a) Representative gating schema for detection of specific IFN-γ induction after peptide stimulation in flow cytometric analysis. Data on PBMCs of macaque VW4 at week 38 without stimulation (NC) and with stimulation using overlapping peptides spanning the N-terminal half of Gag proteins (Gag) are shown. (b) SIV antigen-specific CD8+ T-cell frequencies at indicated time points after SIVmac239 infection. CD8+ T-cell responses targeting SIV Gag, Vif, Nef, Pol, Vpx/Vpr, Tat/Rev, and Env were examined by detection of specific IFN-γ induction after stimulation using overlapping peptides spanning individual antigens. Log-transformed CD8+ T-cell frequencies are shown. PBMCs were obtained at weeks 2–12 (before ART initiation), 26 (just before the 1st vaccination), 27 (1 week after the 1st vaccination), 34 (2 weeks after ART cessation) and 38 post-infection and subjected to the analyses. ND not determined because of the limitation of available samples.

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