Figure 2
Protein starvation impairs ubiquitin–proteasome activity in testis muscle. (A–B’) Testes from mef2 > CL1-GFP flies immunostained for GFP (green), co-stained for N-cadherin (red) and DNA (blue). Anti-N-cadherin staining labels the muscle membrane. Flies were either fed (A,A’) or starved (B,B’) for proteins for 18 days. (C) The percentages of testes showing CL1-GFP accumulation in the testicular muscles. Testes were from males either fed or starved for 6, 15, 18 and 21 days. (D,E) GFP expressed from Egr-GFPv in testes of mef2 > DTS5 DTS7 males. (D) The percentage of testes showing GFP accumulation in the muscles. Fed flies were maintained at 29 °C to enhance DTS5 and DTS7 expression for 15, 18 or 21 days as indicated. (E) Testes immunostained for GFP. Upregulation of Egr-GFP was not detected in testicular muscle until starved for 9 days. Adult flies were maintained at 29C during the starvation phase.
