Figure 4
Egr is required for fast recovery of CySC number upon protein refeeding. (A–F) Testes immunostained for Zfh-1 (green), co-stained for Vasa (red) and FasIII (blue). (A,C,E) Testes from males starved for 18 days (S18). (B,D,F) Testes from males starved for 18 days and refed for two days (S18R2). (G–I) Average number of CySCs during refeeding phase. Data are presented as mean ± SD. The CySC recovery rate at S18 and S18R5 is set as 0% and 100%, respectively. The recovery rate at R2 is indicated (see “Methods”). (G) CySC recovery in Oregon R (OR), w1118 and egr1 adults upon refeeding. Number of testes for each time point is between 11 and 36. (H) Lack of Grnd in grndminos/Df (BSC149) mutant delayed CySC recovery upon refeeding. The number of testes for each point is between 25 and 38. (I) Tissue-specific depletion of egr in testicular muscle delayed CySC recovery upon refeeding. The number of testes for each point is between 19 and 37. (J) Quantification of food consumed by adult flies after starvation for 18 days (S18) and 1-day refeeding (S18R1). Statistics were generated by Student t test.
