Figure 1 | Scientific Reports

Figure 1

From: LncRNA RP11-86H7.1 promotes airway inflammation induced by TRAPM2.5 by acting as a ceRNA of miRNA-9-5p to regulate NFKB1 in HBECS

Figure 1

Effects of TRAPM2.5 on the inflammatory response of human bronchial epithelial cells. (A) ELISAs analysis of IL-6, IL-8, and TNF-α levels in HBECs treated with 0, 50, and 100 μg/ml TRAPM2.5 for 48 h. (B) Western blotting analysis of Iκκβ, p-IκB-α, IκB-α, p-p65 and p65 in HBECs treated with 0, 50, and 100 μg/ml TRAPM2.5 for 48 h. GAPDH was used as an internal control. (C) Immunofluorescence microscopy showing the localization and expression of p65 in HBECs treated with 0, 50, and 100 μg/ml TRAPM2.5 for 48 h. Original magnification, × 200. Scale bar 20 μm. The data are shown as the means ± standard deviations (n = 3). The statistical significance of the data was assessed by Student’s t test. *P < 0.05, **P < 0.01.

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