Figure 4 | Scientific Reports

Figure 4

From: LncRNA RP11-86H7.1 promotes airway inflammation induced by TRAPM2.5 by acting as a ceRNA of miRNA-9-5p to regulate NFKB1 in HBECS

Figure 4

Full-length sequence, coding ability, subcellular distribution and coexpression network characteristics of the lncRNA RP11-86H7.1. (A) 5′-RACE and 3′-RACE assays were performed to determine the transcriptional initiation and termination sites of the lncRNA RP11-86H7.1. Left, representative images of the PCR products obtained by 5′-RACE and 3′-RACE. Right, the sequence of the second-round PCR products revealed the boundary between the universal anchor primer and the lncRNA RP11-86H7.1 sequence. (B) FISH analysis of the subcellular distribution of the lncRNA RP11-86H7.1 in 16HBE cells. Scale bar 20 μm. (C) Co-expression network characteristics of the lncRNA RP11-86H7.1. FAM refers to carboxyfluorescein, FAM-labeled lncRNA RP11-86H7.1 was stained green (FAM), and nuclei was stained blue with 6-diamidino-2-phenylindole (DAPI).

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