Figure 6 | Scientific Reports

Figure 6

From: LncRNA RP11-86H7.1 promotes airway inflammation induced by TRAPM2.5 by acting as a ceRNA of miRNA-9-5p to regulate NFKB1 in HBECS

Figure 6

LncRNA RP11-86H7.1 acts as a ceRNA and competitively absorbs miR-9-5p with NFKB1. (A) LncRNA RP11-86H7.1, miR-9-5p and NFKB1 binding sites predicted by miRanda. (B) The luciferase activities in 293 T cells co-transfected with wild-type (wt) and mutant (mut) NFKB1 or RP11-86H7.1 plasmid together with miR-9-5p mimic or miR-9-5p negative control (NC). Luciferase activity was detected 24 h after transfection using the dual luciferase assay. Data are presented as the relative ratio of renilla luciferase activity and firefly luciferase activity. (C) The real-time PCR results of the RIP based on Ago2 showed that lncRNA RP11-86H7.1 can compete with the NFKB1 for the binding of miR-9-5p. IgG was used as negative control. The data are shown as the means ± standard deviations (n = 3). The statistical significance of the data was assessed by Student’s t test. *P < 0.05, **P < 0.01.

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