Figure 2 | Scientific Reports

Figure 2

From: Analysis of tumor mutational burden: correlation of five large gene panels with whole exome sequencing

Figure 2

(a) TMB values of the panels (x-axis) compared to results from paired tumor-normal WES of non synonymous variants (y-axis). If bioinformatic pipelines delivered results including coding synonymous variants as well as excluding them, the ones with the highest correlation to the tumor-normal paired analysis are shown. Panels: Illumina—TSO500; NEO—NEOplus v2 RUO TMB (NEO New Oncology); Agilent custom—custom panel Agilent SureSelect XT HS; Exome tumor only—WES Agilent SureSelect XT HS Human All Exon v6 panel; Qiagen Mutect2—Qiagen TMB v3.0 (Qiagen) analyzed with Mutect2 in-house pipeline; Qiagen Genomics—Qiagen TMB v3.0 (Qiagen) analyzed with Qiagen Genomics Workbench 12.0.2.; Thermo—Oncomine Tumor Mutation Load assay (Thermo Fisher Scientific); syn.—Analysis includes synonymous variants. (b) Average normalized TMB values (y-axis) for all panels (x-axis) shown as violin plots. The red dots are the samples for the panels. The outer shape represents the density distribution and filling heat map corresponds to the Pearson correlation to exome t-n. The lower and upper hinges correspond to the first and third quartiles (the 25th and 75th percentiles) with 95% confidence interval.

Back to article page