Table 1 Comparison of the five TMB panels.

From: Analysis of tumor mutational burden: correlation of five large gene panels with whole exome sequencing

 

Oncomine tumor mutation load assay (thermo fisher scientific)

TruSight oncology 500 assay (illumina)

NEOplus v2 RUO panel (NEO new oncology)

SureSelect XT HS custom TMB panel (agilent)

QIAseq TMB panel (qiagen)

DNA input (ng)

20

40

200/100*

10–200

40

Technology

Amplicon-based

Hybridization-based

Hybridization-based

Hybridization-based

Single primer extension

Unique molecular identifier

No

Yes

No

Yes

Yes

Genes

409

523

340

362

486

Targeted panel size (Mb)

1.7

1.9

2.5

3.1

1.3

Targeted panel size—coding region (Mb)

1.2

1.24

1.2

1.1

1.3

  1. Comparison of features for the different gene panels; only coding regions were used in the analysis.
  2. *for explanation, see “Methods” section.
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