Figure 1 | Scientific Reports

Figure 1

From: Identification of new regulatory genes through expression pattern analysis of a global RNA-seq dataset from a Helicobacter pylori co-culture system

Figure 1

Helicobacter pylori co-culture alters macrophage transcriptomic profile, leading to the activation of early regulatory responses and increasing bacterial persistence in WT cells. WT and PPARγ-deficient BMDM were co-cultured ex vivo with H. pylori and cells were harvested at several time-points ranging from 0 to 720 min after gentamycin treatment. Bacterial burden (A) and gene expression, including IFNγ (B) and IL-10 (C) were assessed. To classically activate macrophages prior to H. pylori co-culture, cells were stimulated with LPS and IFNγ, then challenged with H. pylori and bacterial burden was measured (D). Differential gene expression based on genotype (E) and H. pylori infection (F) from a whole transcriptomic analysis performed on the harvested cells were also assessed. The red arrow highlights the 120 min timepoint that corresponds to the peak of bacterial growth. *P < 0.05 between genotypes, #P < 0.05 within each genotype compared to time 0.

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