Figure 2 | Scientific Reports

Figure 2

From: Identification of new regulatory genes through expression pattern analysis of a global RNA-seq dataset from a Helicobacter pylori co-culture system

Figure 2

Initial analysis and validation of the whole transcriptomic analysis revealed two genes with differential expression pattern and well-defined anti-microbial functions. Plots represent RNAseq reads of Chil1 (A), Iigp1 (B), and Sqle (C) during the entire time-course comparing genotypes and treatments. Chil1 (D), Iigp1 (E), and Sqle (F) gene expression from the same co-culture was validated through qRT-PCR. Bacterial loads (G) were measured 120 min post-gentamycin treatment of H. pylori co-cultures in WT and PPARγ-deficient macrophages transfected with Chil1-targeted, Iigp1-targeted or Sqle-targeted siRNA or a scrambled sequence as a negative control. *P < 0.05 between genotypes, #P-value < 0.05 within each genotype compared to time 0.

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