Figure 7 | Scientific Reports

Figure 7

From: Identification of new regulatory genes through expression pattern analysis of a global RNA-seq dataset from a Helicobacter pylori co-culture system

Figure 7

In vivo validation of Plxdc2 regulatory role in a murine model of H. pylori infection. WT and Plxdc2−/− mice were infected with H. pylori SS1. Stomach and GLN were collected at day 28 post infection. Bacterial burden (A) and iNOS (B) expression were measured in gastric tissue. GLN-isolated cells were stimulated ex vivo with inactivated H. pylori SS1 for 72 h. Secreted TNFα (C), MCP1 (D) and IL-17 (E) were quantified 72 h post culture through cytokine bead array. WT and Plxdc2−/− mice were infected with H. pylori PMSS1. Representative images of hematoxylin and eosin-stained gastric sections WT and Plxdc2−/− mice infected with PMSS1 at day post infection 28 (F). These images were obtained using the Olympus CellSens Entry imaging software. Averaged histopathological scores of leukocyte infiltration (G) and mucosal hyperplasia (H) *P < 0.05.

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