Figure 2 | Scientific Reports

Figure 2

From: Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits

Figure 2

ALA-SDT induces macrophage apoptosis and reduces TNF-α, IL-6 and IL-1β expression in the media of balloon-denuded rabbit femoral arteries at day 1 after treatment. (A) Rabbit tissue Western blot analysis of apoptosis-related proteins, including pro-caspase 3, cleaved caspase-3 and Bcl-xl. GAPDH was used as a loading control. (B) Immunofluorescence staining of TUNEL and RAM-11 to assess the macrophage apoptosis rate in the indicated groups (n = 6). Scale bar = 100 μm. (C) Representative sections of TNF-α, IL-6 and IL-1β expression stained by immunohistochemistry and quantitative analysis in the indicated groups (n = 7). Scale bar = 200 μm. Each data point represents one rabbit. *p < 0.05, **p < 0.01 and ***p < 0.001 versus control.

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