Figure 1
Preliminary tests for a MT medium throughput screening assay based on transgenic C. elegans. (a) Fluorescence of MT::gfp in strains CL2122 and CL2120 was measured by a fluorescence multiwell plate reader at em/ex 450/535. (b) Fluorescence in strains CL2120 and CL2122 was detected at day 6 by fluorescent microscope (magnification ×20). (c) Serial dilution (1:1) of CL2120 L4 larvae in a 96 well plate starting with 512 worms/well in triplicates. Fluorescence of reporter was measured after 3, 6 and 9 days. (d) Serial dilution (1:1) of Escherichia coli strain OP50 in a 96 well plate starting with a concentration of 6 mg/ml. Absorbance was detected by a multiwellplate reader at 600 nm. OD600 = 0,8 at a concentration of 5 mg/ml. (e) Influence of different DMSO concentrations on fluorescence of GFP reporter in strains CL2120, CL2659 and NL5901. *p ≤ 0.05; **p ≤ 0.005.
