Table 2 Selected distances between the two zinc ions in the active site of MIM-1 and SAM-1, and their ligands.

From: Structure and mechanism of potent bifunctional β-lactam- and homoserine lactone-degrading enzymes from marine microorganisms

Interaction partner

MIM-1 (Å)

SAM-1 (Å)

Zn1 (α site)

 

His116

2.09

2.20

His118

1.91

2.03

His194

2.08

2.03

W1

–

1.96

W2

–

3.07

O1 (displaced W1)

2.58

–

O3 (displaced W3)

2.70

–

Zn2 (β site)

  

Asp120

2.34

2.03

His121

1.97

2.21

His260

2.00

2.10

W1

–

1.99

W2

–

2.02

O4 (displaced W1)

1.86

–

O1 (displaced W2)

2.18

–

Zn1–Zn2

4.02

3.40

  1. W represents water molecules and O oxygen atoms of the citrate molecule bound to MIM-1.
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