Figure 6
Loss of p53 permits centrosome amplification in the setting of MCPH1 depletion. (a) MCPH1 shRNA was used to deplete MCPH1 in, DLD1, HCT116 p53+/+, and HCT116 p53−/−, and HeLa cells. Scrambled shRNA was used as a control. (b) Quantification of centrioles per cell in the cells treated with shRNA. Each dot represents a single cell, color coded by each independent experiment, with mean values for each experiment indicated by larger, outlined dots. Bars represent mean ± SEM from 3 independent experiments. (c) Quantification of CA, defined as the percent of cells with greater than 4 centrin foci. Each dot represents a single experiment, bars represent means ± SEM. Vertical dotted lines are shown to separate cells based on p53 status; DLD1 and HCT p53+/+ have wild-type p53, and HCT116 p53−/− and HeLa lack functional p53. *P value < 0.05, ***P value < 0.001.
