Figure 2
From: 1.2 MV/cm pulsed electric fields promote transthyretin aggregate degradation
Transthyretin aggregate disappeared after exposure to 1,000 pulses at 1.26 MV/cm. (A) Aggregate of WT and L55P mutants disappeared after being exposed to 1,000 pulses at 1.26 MV/cm. Aggregate concentrations during PEF treatment were 0.2 mg/mL. NC was an aggregate solution (0.4 mg/mL) mixed with PEF-treated triple-diluted HEPES buffer at a volume ratio of 1:1. 1 µg of aggregate was in the native PAGE gels. Aggregate distributions of WT (B) and L55P mutants (C) of 0 pulse and 1,000 pulses at 1.26 MV/cm. The full photograph without contrast of gel electrophoresis is in SI Fig. S1B. (D) Aggregate fluorescent photos with a green fluorescence filter. Application of 1,000 pulses at 1.26 MV/cm reduced fluorescent dots. The white scale bar represents 100 μm. Each experiment was performed more than twice. Image J adjusted (A,D), and gained the distributions of (B,C).
