Figure 5
From: 1.2 MV/cm pulsed electric fields promote transthyretin aggregate degradation
Aggregate-derived transthyretin subunit analysis with SDS-PAGE. Application of 1,000 pulses at 1.26 MV/cm destroyed aggregate-derived transthyretin subunits and weaker electric fields (A), whereas fewer pulses at 1.26 MV/cm decreased the ability (B) in both WT and L55P mutants. NC is negative control. The aggregate concentration during PEF treatment was 0.2 mg/mL in A and B. Aggregate-derived subunit band-intensity distributions of WT (C) and L55P mutants (D) of 0 pulse and 1,000 pulses at 1.26 MV/cm. 0.35 μg of aggregate in the gels. The full photographs without contrast of gel electrophoresis are in SI Fig. S5. (E) Comparing the absorbance of aggregates using BCA, the L55P mutant exhibited a significant decrease after 1,000 pulses at 1.26 MV/cm but WT proteins did not (n.s means no significant, *p < 0.01, N = 3). Image J adjusted (A,B), and gained the distributions of (C,D).
