Figure 6
From: 1.2 MV/cm pulsed electric fields promote transthyretin aggregate degradation
Tetramer-derived transthyretin subunit analysis with SDS-PAGE. (A) 1,000 pulses at 1.26 MV/cm slightly reduced the tetramer-derived subunit band. NC is negative control. Tetramer-derived subunit band-intensity distributions of WT (B) and L55P mutants (C) of 0 pulse and 1,000 pulses at 1.26 MV/cm. The tetramer concentration during PEF treatment was 0.1 mg/mL in A. The analysis in (A) was performed twice with SDS-PAGE. A 0.35-μg sample of transthyretin was in the gels. The full photographs without contrast of gel electrophoresis are in SI Fig. S6. (D) Center-position intensity ratios of tetramer- or aggregate-derived subunits of WT and L55P mutants (*p < 0.01, N = 10). Details for calculating the ratios are supplied in SI Fig. S6C. Image J adjusted (A), and gained the distributions of (B,C).
