Figure 1
(A) In situ liver photographs from control and DON-treated animals. (B) Representative microphotographs of frozen liver section stained with ORO observed in vehicle (distilled water) or 12.5 mg/kg DON for the indicated times. High magnifications of ORO staining observed 6 h after vehicle or 12.5 mg/kg administration. Areas where these high magnifications originate are visualized by square in 6 h photographs. Scale bars = 100 and 30 µm in low and high magnification respectively. (C, D) Quantification of ORO positive inclusion number per surface unit (C) and size (D) in liver of vehicle and DON-treated mice. (E) Representative Western blot and quantitative comparison of ADRP protein expression (48 kDa) assessed in vehicle and DON-treated mice. GAPDH expression was used to normalize the results (not shown). NQ indicates non quantifiable data. A two-way analysis of variance test was performed between time series (3, 6 and 12 h) of DON-treated (12.5 mg/kg bw) and untreated mice (panels C, D and E). *P < 0.05 and **P < 0.01 significantly different from control mice. #P < 0.05 and ##P < 0.01 significant difference between time point of DON-treated mice. ns: no significant difference.
