Figure 2
(A, B) Acute p.o. DON administration modifies food intake. Cumulative food intake (A) and food intake measured at different time intervals (B) over a 24 h period in mice p.o. administered either vehicle or DON (12.5 mg/kg bw). (C, D) DON-induced hypoglycemia. Time course of blood glucose concentration expressed in mg/dL (C) and normalized or DON (12.5 mg/kg bw, blue line, (D). Insets in (C) Area under the cure calculated for two time periods i.e. 0–12 h and 0–24 h. (E, G) Dosages of plasma insulin (E), adrenaline (F) and corticosterone (G) concentrations assayed for vehicle- and DON-treated animals. (H) mRNA expression of Pck1 assessed by qRT-PCR from the livers of control and DON-challenged animals for the indicated times. A two-way analysis of variance test was performed between time series (3, 6 and 12 h) of DON-treated and untreated mice (panels A, B and E–H). Comparisons between data from vehicle and DON-treated mice were performed using unpaired 2-tailed Student’s t test (panels C, D). *P < 0.05 **P < 0.01 and ***P < 0.001 significantly different from control mice. #P < 0.05, ##P < 0.01 and ###P < 0.001 significant difference between time point of DON-treated mice.
