Table 1 Primers sequences used for SYBR Green assays.
Gene product | RefSeq | Primers | |
|---|---|---|---|
Fw | Rv | ||
Acaca | NM_133360.2 | GGGAACATCCCCACGCTAAA | GAAAGAGACCATTCCGCCCA |
Acadm | NM_007382.5 | CACAACACTCGAAAGCGGC | GATGAGAGGGAACGGGTACTC |
Acox1 | NM_015729.3 | TCACAGCAGTGGGATTCCAAA | CGGCAGGTCATTCAAGTACG |
Atf6 | NM_001081304.1 | ACGAGGTGGTGTCAGAGAAC | GCAGGGCTCACACTAGGTTT |
Cact | NM_020520.5 | CAGATTCAGGCTTCTTCAGGG | CCACTGGCAGGAACATCTC |
CHOP | NM_007837.4 | CCCCAGGAAACGAAGAGGAA | GACTGGAATCTGGAGAGCGA |
Cpt1 | NM_153679.2 | TGCTGTCTTCACTGAGTTCC | CACCAGCAAGAAAGTCATTCCA |
Cpt2 | NM_009949.2 | ACAGCATCGTACCCACCAT | ACACAACACTTCTGTCTTCCTGA |
Cyp4a10 | NM_010011.3 | TCCCAAGTGCCTTTCCTAGA | GGGGTTAGCATCCTCCTGT |
Fasn | NM_007988.3 | GCCTCACTGCCATCCAGATT | CCCAAGGAGTGCCCAATGAT |
Hmgcr | NM_008255.2 | GTCGCTGGTCCTAGAGCTT | CCTTTGGGTTACGGGGTTTG |
IL-1β | NM_008361.3 | GACCCCAAAAGATGAAGGGCT | AAGGTCCACGGGAAAGACAC |
IL-6 | NM_031168.2 | ACAACCACGGCCTTCCCTAC | ATCAGAATTGCCATTGCACAAC |
Ire1 | NM_023913.2 | ACCTGCCCAAACATCGAGAA | CCTCCACATCCTGAGATACGG |
HSL | NM_010719.5 | CCTGGAACTAAGTGGACGCA | ACATCTCGGGGCTGTCTGAA |
MGL | NM_001166251.1 | CTCCACAGAATGTTCCCTACCA | TGTCCAGCCCCTTCAACATA |
PEPCK | NM_1044.3 | ACTGACAGACTCGCCCTATGTG | GTTGCAGGCCCAGTTGTTGA |
Perk1 | NM_010121.3 | AGAACAGCGTGTACTTAGGGA | AGACCCAACCAAGACAGGA |
ATGL | NM_025802.3 | TCCAACATGCTACCAGTGCG | AGCCACTCCAACAAGCGG |
Scd1 | NM_009127.4 | CAAGCTGGAGTACGTCTGG | GCGCTGGTCATGTAGTAGAA |
Srebf1 | NM_011480.4 | ACTCCCTCTGATGCTACGG | TGGGTCCAATTAGAGCCATCTC |
Srebf2 | NM_033218.1 | GCGACCAGCTTTCAAGTCC | CATTGGCTGTCTGCGTCAAC |
TNFα | NM_013693.3 | TCTTCTCATTCCTGCTTGTGG | GAGGCCATTTGGGAACTTCT |
XBP1 | NM_001271730.1 | CTGGCGTAGACGTTTCCTGG | GCCTTACTCCACTCCCCTTGG |
GAPDH | NM_001289726.1 | TTCTCAAGCTCATTTCCTGGTATG | GGATAGGGCCTCTCTTGCTCA |
POLR2a | NM_001291068.1 | GCACCATCAAGAGAGTGCAG | GACCTCCCTCCGTTGTTTC |
U6 | NR_003027.2 | GCTTCGGCAGCACATATACTAAAAT | CGCTTCACGAATTTGCGTGTCAT |
YWASZ | NM_001253805.1 | ACATCTGCAACGATGTACTGTCT | TGCTGTGACTGGTCCACAAT |
