Figure 5
From: Brown adipose tissue ameliorates autoimmune arthritis via inhibition of Th17 cells
Comparison of gene expression between normal BAT and CIA BAT in mice by microarray analysis. (A) Hierarchical cluster heatmap of normal BAT and CIA BAT. (B) Scatter plots of the expression levels of genes between normal BAT and CIA BAT. (C) Significantly different gene numbers are shown in normal BAT and CIA BAT. (D,E) Top 10 biological processes and molecular functions associated with upregulated and downregulated genes in normal BAT and CIA BAT, based on gene ontology functional analysis. (F) The relative expression levels of total PI3K (PI3K), phosphorylated PI3K (p-PI3K), total AKT (AKT) and phosphorylated AKT (p-AKT) proteins were determined by western blotting. (G) The protein expression levels which were the ratio of the phosphorylated protein/total protein values of PI3K and AKT were monitored. *P < 0.05, **P < 0.01, and ***P < 0.005.
