Figure 5
From: Alteration of immunophenotype of human macrophages and monocytes after exposure to cigarette smoke
Effects of a combination of CSE and IL-4 on M2 cytokine production and expression by MDMs. The MDMs were incubated with medium alone (control), 4% CSE, 10 ng/mL IL-4 or 4% CSE + IL-4 10 µg/mL for 24 h. The culture supernatants were collected and the concentrations of MDC/CCL22, TARC/CCL17, IL-10, and PARC/CCL8 were measured using an ELISA (a–d). mRNA expression was then determined using RT-PCR (e–i). The results were normalized against expression of the GAPDH gene. The data were assessed in an ANOVA, followed by a Bonferroni post-test. The data correspond to the mean ± SEM of 9 donors. *p < 0.05 compared with the control; αp < 0.05, compared with 4% CSE; #p < 0.05 compared with 10 ng/mL IL-4.
