Table 2 Dilution used for specific cytokines for each conditions.
From: Alteration of immunophenotype of human macrophages and monocytes after exposure to cigarette smoke
Cytokine | MDM | Monocytes | ||||||
|---|---|---|---|---|---|---|---|---|
Control | CSE 4% | LPS | LPS + CS4% | IL-4 | IL-4 + CS4% | Control | LPS | |
Dilution | Dilution | |||||||
IL-6 | 1:1 | 1:1 | 1:200 | 1:200 | 1:1 | 1:1 | 1:2 | 1:50 |
TNF-α | 1:1 | 1:1 | 1:100 | 1:100 | 1:1 | 1:1 | 1:1 | 1:10 |
IL-8/CXCCL8 | 1:10 or 1:100 | 1:100 | 1:100 or 1:200 | 1:100 | 1:100 | 1:100 | 1:100 | 1:100 |
Gro-α/CXCL1 | 1:1 | 1:1 | 1:100 | 1:100 | 1:1 | 1:1 | 1:2 | 1:10 |
MCP1/CCL2 | 1:4 | 1:4 | 1:20 | 1:20 | 1:4 | 1:4 | - | - |
MDC/CCL22 | 1:100 | 1:100 | 1:100 or 1:200 | 1:100 or 1:200 | 1:100 or 1:200 | 1:100 or 1:200 | 1:100 | 1:100 |
IL-10 | 1:1 | 1:1 | 1:1 | 1:1 | 1:1 | 1:1 | - | - |
PARC/CCL18 | 1:10 | 1:10 | 01:10 | 01:10 | 01:10 | 1:10 | - | - |
TARC/CCL17 | 1:1 | 1:1 | 1:1 | 1:1 | 1:1 | 1:1 | 1:2 | 1:2 |
