Figure 3

Epimerase activity of AcAlgE1 as a function of calcium. Mannuronan was epimerized for 1 h (filled) and 19 h (cross-hatched) with AcAlgE1 at different calcium concentrations before the G-lyase AlyA was added and the incubation continued for two hours. The activity is given as the increase in absorbance at 230 nm.