Figure 2
GFP-LVS replicates to high numbers in murine BMM, and LVS remains cytosolic but not associated with vacuoles. Murine BMM were cultured ± 50 ng/ml recombinant mouse IFN-γ for 24 h, as indicated, and then infected with GFP-LVS at an MOI of 50:1. (A) Representative images of GFP-LVS-infected (green) macrophages (not treated with IFN-γ) with the indicated trafficking markers (red) 48 h after infection. Macrophage nuclei were stained with DAPI (blue). Scale bar = 50 µM. (B) Colocalization of single GFP-LVS bacteria with markers, as illustrated in (A), was quantified at the indicated time points after infection. At least 70 bacteria were scored from two identical replicates for each condition. Results shown are combined means ± SD for three independent experiments. No significant (P > 0.05) or consistent differences were found between any combination of groups.
