Figure 4

Administration of recombinant progranulin suppressed the infiltrating neutrophils after myocardial infarction in mice. (A) Reparative images show immunostaining for NIMP-R14 at the infarct and border areas 1 day after permanent occlusion of LCA. Nuclei were stained with Hoechst 33342. (B) Number of neutrophils per mm² at infarct area after permanent occlusion of LCA. (C) Number of neutrophils per mm² at border area after permanent occlusion of LCA. The administration of recombinant progranulin reduced the number of infiltrating neutrophils at the infarct area compared with vehicle-treated group. Scale bar: 50 μm. Data are the means ± SEM. (n = 5–6) ^*p < 0.05, ^**p < 0.01 versus sham-operated group (two-tailed Student's t-test), ^†p < 0.05 vs. vehicle-treated group (one-tailed Student's t-test).