Figure 5 | Scientific Reports

Figure 5

From: Dual SMAD inhibition and Wnt inhibition enable efficient and reproducible differentiations of induced pluripotent stem cells into retinal ganglion cells

Figure 5

Functional characteristics of induced retinal ganglion cells. (A) RGCs were transduced with AAV7m8-SNCG-eGFP and immunofluorescence RGC markers (BRN3A, THY1, TUBB3, MAP2, TUJ1, RBPMS). (B) Density of RGCs expressing GFP and other axonal processes increases between DIV35 (Fig. 4B, cell c) and maturation DIV75 (Fig. 4B, cells a and b). Blue arrows point to cells selected for patch clamp recordings and that fired action potential upon depolarization. (C) Action potential firing in response to depolarizing voltage step recorded from cell a and cell b (DIV75) and from cell c (DIV35). (D) This trace demonstrates action potential firing in response to the two different depolarizing steps of current recorded from cell a from Fig. 4B. We also observe a spontaneous spike firing at the start of the trace. Same scale bar applies to all images.

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