Figure 2
From: Inhibition of mitotic kinase Mps1 promotes cell death in neuroblastoma
Mps1 inhibition kills neuroblastoma cells. (A) Neuroblastoma cells were treated for 72 h with DMSO as control, 0.3 μM reversine or 1 μM Mps-BAY2a and then co-stained with the vital dye propidium iodure (PI) and the mitochondrial membrane potential (Δψm)-sensing dye DiOC6(3) for the evaluation of cell death-associated parameters by cytofluorometry. White and black columns depict the percentage of dying (PI−DiOC6(3)low) and dead (PI+) cells, respectively. (B–D) SK-N-Be2c neuroblastoma cells were transfected with an unrelated siRNA (siUNR) or three specific siRNAs directed against Mps1 (siMps1a, siMps1b and siMps1c). Upon 72 h, cells were collected and lysed for real time PCR analysis (B). Alternatively, cells were subjected to the determination of the cell death–associated parameters by flow cytometry upon co-staining with the propidium iodide (PI) and DiOC6(3) dyes. Representative plots are showed in panel (C) while quantitative data are displayed in panel (D). White and black columns illustrate the percentage of dying (PI− DiOC6(3)low) and dead (PI+) cells, respectively. Data are reported in SEM n = 3. **(p < 0.01) and ***(p < 0.001) indicates significant difference from the DMSO control treatment or siUNR transfected cells (ANOVA).
