Figure 5

Neighbor-joining phylogenetic tree based on partial 16S rRNA gene sequences illustrating the phylogenetic affiliation of the assigned OTUs (blue colour) within the genus Legionella. The 1,000 resampling bootstrap values (%) are indicated at the nodes; only values greater than 50 are shown. Aquicella lusitana is used as an outgroup. The scale bar represents 0.02 substitutions per base position. The sequences derived from cooling tower water in Japan18 are shown in red colour.