Figure 3

Susceptibility of GhABP19-silenced cotton plant. (a, b) Disease symptoms of TRV:00 and TRV:GhGLP2 cotton plants infected by Verticillium dahliae and Fusarium oxysproum 21 days post inoculation. (c) Mean expression levels of GhGLP2 in VIGS cotton plants. Total RNA was extracted from the leaves of 2-week VIGS cotton. Data represent the means ± SD of three independent biological samples with three technical replicates. Student’s t-test, *P < 0.05, **P < 0.01 compared to control plants. (d) Disease index of TRV:00 and TRV:GhGLP2 cotton plants at the indicated days after inoculation with V. dahliae and F. oxysporum, respectively. Error bars represent the standard error of three biological replicates (n ≥ 30). (e) Trypan blue staining of TRV:00 and TRV:GhGLP2 cotton leaves at 48, 72 and 96 h post-inoculation (hpi) with V. dahliae (left panel) and F. oxysporum (right panel). The experiments were repeated three times (n ≥ 10).