Figure 7

Oxidative stress tolerance and H₂O₂ accumulation in wild-type (WT) and GhGLP2-transgenic Arabidopsis plants. Seedling greening ratios (a) and primary root length (b) of WT and GhGLP2-transgenic Arabidopsis plants grown for 10 days on MS medium containing a range of concentrations (0, 0.3, 0.6, 1.0, 1.5 μmol/L) of methyl viologen (MV). (c, d) Effect of ammonium persulfate (APS) treatment under in planta condition. Leaves from uninoculated Arabidopsis plants were treated with 10% APS for 3, 6 and 9 h. (e–h) Histochemical detection of H₂O₂ in Arabidopsis plants by DAB staining (e, f) and H₂O₂ content analysis (g, h) at 48, 72 and 96 h post-inoculation (hpi) with Verticillium dahliae or Fusarium oxysporum. Data represent the means ± SD of three independent biological replicates (n ≥ 10). Student’s t-test, *P < 0.05, **P < 0.01 compared to control plants. Bar = 500 μm.