Figure 3
From: Trisk 95 as a novel skin mirror for normal and diabetic systemic glucose level
High extracellular glucose concentrations induce changes in the intracellular calcium in primary keratinocytes. (a) Intracellular calcium levels in primary keratinocytes after 45 min of glucose treatment. Cells were incubated for 30 min in the presence of Fluo-4 AM and intracellular calcium basal levels were measured for 50 s, after which thapsigargin was added to the medium. Time lapse recording was used to follow the increase in the fluorescence signal until the maximum amplitude was reached. The data showed a significant increase in the amount of cytosolic calcium in the cells incubated with high glucose relative to the controls (n = 41). (b) Speed required to reach the half maximum of the fluorescence signal. The keratinocytes incubated with high glucose were slower to reach their peak than were the controls. The two-tailed Student’s t-test was used for statistical analysis in (a) (*P < 0.01) and (b) (*P < 0.02).
