Figure 1 | Scientific Reports

Figure 1

From: Topical cell-free conditioned media harvested from adipose tissue-derived stem cells promote recovery from corneal epithelial defects caused by chemical burns

Figure 1

Characteristics of cultured rat adipose tissue-derived stem cells (ADSCs). Use of DMEM without growth factors resulted in a relatively large and round epithelial morphology of rat ADSCs (A), whereas use of DMEM/F12 medium with growth factors resulted in relatively small cells with a fibroblast-like spindle shape (B). After 3 weeks of mesenchymal differentiation induction, various lineage differentiations, including fat (C), cartilage (E), and bone (G), was observed, which were stained with Oil Red O (D), Alcian Blue (F), and Alizarin Red S (H), respectively. Surface markers were examined using flow cytometry to define mesenchymal-origin stem cells. Subcultured rat ADSCs were negative for CD31 (I), CD34 (J), and CD45 (K), but were 32.6%, 86.5%, and 24.2% positive for CD73 (L), CD90 (M), and CD105 (N), respectively. Scale bar, 100 μm; ADSCs, adipose tissue-derived stem cells; Scale bar, 100 μm.

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