Figure 6

Immunohistochemistry of corneas harvested from the CM, CB, and NC groups. Paraffin section slides of both groups were stained for PCNA (A–D), Iba-1 (E,F), or ZO-1 (G,H) and with DAPI. Cells stained with antibodies and DAPI were counted and compared for each group. The representative immunohistochemically stained pictures of PCNA are shown in (I) and (J), which were stained at the corneal center of the CB and CM groups, 3 days after induction of chemical burn, respectively. The representative images of Iba-1 are shown in (K) and (L) of CB and CM groups in the limbal area one day after induction of chemical burn, respectively. The representative pictures of ZO-1 on the third day are shown in M and N of the central epithelium of the CB and CM groups, respectively. The epithelium layer is marked by a black arrowhead. PCNA, proliferating nuclear antigen; Iba-1, ionized calcium binding adaptor molecule-1; ZO-1, zona occludens-1; scale bar, 100 μm.