Figure 4
From: Intracellular interplay between cholecystokinin and leptin signalling for satiety control in rats
Cytosolic Ca2+ mobilization in the VMN. (A) VMN cells were repeatedly stimulated by 500 nM leptin and 10 nM CCK-8s. Three representative cell responses are shown. Note that there are three populations that displayed increased Ca2+ solely by leptin (black) or CCK-8s (blue) or by both (pink). (B) Left: representative cell responses following co-stimulation with 100 nM leptin and 1 nM CCK-8s. Right: Representative cell responses following stimulation with 100 nM leptin under the treatment of 1 µM LGM. (C) Concentration response curves for leptin (Rmax = 0.2, EC50 = 152.9 nM). The response curve was shifted up by co-stimulation of 1 nM CCK-8s (Rmax = 0.26, EC50 = 42.8 nM) and shifted down by blocking CCK-1 receptors by 1 µM LGM (Rmax = 0.14, EC50 = 162.7 nM). **p < 0.01 by two-way ANOVA.
