Figure 4
From: Cardioprotective effects of miR-34a silencing in a rat model of doxorubicin toxicity
Ant34a regulates myocardial apoptosis and senescence in DOXO treated rats. (a) Cardiac apoptosis measured by TdT assay. (b) Cardiac apoptic cells within the myocardium of DOXO (left panel, TdT green) and Ant34a treated (central panel, TdT green; right panel, TdT red) animals. Cardiomyocytes are labelled with α-SA (red), nuclei are stained with DAPI (blue). Scale bars 20 μm. (c) Levels of acetyl-p53Lys381 were evaluated by western blot analysis. (d) p16INK4a expression was examined by western blot analysis. (e) Representative images of western blot protein bands. Full-length blots are displayed in Supplementary Fig. S1d–g. Protein expressions are shown as fold change with respect to CTL group. Results are presented as mean ± SD, n = 5–8/group. *p < 0.05 vs CTL; °p < 0.05 vs DOXO; #p < 0.05 vs D + AntCTL. D: DOXO.
