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Figure 1

From: 11-Deoxycortisol controls hydromineral balance in the most basal osmoregulating vertebrate, sea lamprey (Petromyzon marinus)

Figure 1The alternative text for this image may have been generated using AI.

Lamprey corticosteroid biosynthesis and characterization of the lamprey CR. (A) Corticosteroid biosynthetic pathway; dotted box represents the pathway components present in lamprey (cyp11b genes are absent in lamprey). Corticosteroid abbreviations: PREG, pregnenolone; 17P5, 17-hydroxypregnenolone; P, progesterone; 17OHP, 17-hydroxyprogesterone; DOC, deoxycorticosterone; S, 11-deoxycortisol; B, corticosterone; A, aldosterone. Chemical structures were drawn using MarvinSketch Version 20.13 (ChemAxon; https://www.chemaxon.com). (B) Representative receptor binding curves for total binding (BT), nonspecific binding (BNS), and specific binding (Bs = BT − BNS). (C) Representative specific binding saturation curve showing calculated values for corticosteroid receptor abundance (Bmax) and equilibrium dissociation constant (Kd); insert: Rosenthal plot of saturation curve (B = bound, F = free). (D) Relative binding affinity for endogenous (S and DOC) and later-evolved (F and A) corticosteroids (n = 3; values represent mean ± SEM); IC50 (half-maximal inhibition) values: IC50(S) = 15.7 nM; IC50(DOC) = 89.1 nM (unpaired t test: P < 0.001; n = 3).

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