Figure 4 | Scientific Reports

Figure 4

From: 11-Deoxycortisol controls hydromineral balance in the most basal osmoregulating vertebrate, sea lamprey (Petromyzon marinus)

Figure 4The alternative text for this image may have been generated using AI.

Effects of in vivo S treatment on lamprey osmoregulation after SW exposure. Mid-metamorphic sea lamprey were administered S or a vehicle control (Veh), then exposed 12 days post-injection to 25‰ SW for 24 h. (A) Plasma [Cl−], (B) Gill NKA activity. (C,D) gill mRNA abundance of nka and nkcc1. (E,F) Representative bands and quantification of gill NKA and NKCC1 protein abundance. T0 = uninjected FW control sampled at time of S administration. Dose of S is stated in parenthesis (μg g−1 body weight). Values represent mean ± SEM and data points with different letters are significantly different (n = 8–10; one-way ANOVA (A–F: P < 0.001); Tukey’s post hoc).

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