Figure 5

taken from mid-metamorphic sea lamprey incubated ex vivo with varying concentrations of S for 24 h. Dose of S is stated in parenthesis (μg mL−1). Pre = gill tissue prior to incubation; Ctrl = media only. Values represent mean ± SEM and data points with different letters are significantly different (n = 6; one-way ANOVA; Tukey’s post hoc).
Effects of ex vivo S treatment on gill ion transporter expression. Changes in abundance of (A) nka mRNA (P < 0.05) and (B) nkcc1 mRNA (P = 0.24) in gill explants