Figure 8
From: Selection of mutant µplasmin for amyloid-β cleavage in vivo
F587A structural features (a) and the N-terminal fusion and Cys-pegylation positions of μPlm (b). (a) Comparison of water distribution around the active site between WT and F587A mutant, with the inset figure highlighting the multiple water molecules around S741 for F587A mutant. (b) The figure shows that the N-terminal is on the opposite side of the catalytic triad and there is no steric hindrance for connecting a fusion partner to the N-terminal of μPlm (also see Fig. 1a).
