Figure 2 | Scientific Reports

Figure 2

From: The histone lysine methyltransferase SETD8 regulates angiogenesis through HES-1 in human umbilical vein endothelial cells

Figure 2

BVT-948 treatment disrupts actin stress fiber in HUVECs. (A) Immunofluorescence image showing actin stress fiber. HUVECs were treated with BVT-948 (5 μM) in the presence of LSGS supplement for 24 h and stained with FITC-conjugated phalloidin. Scale bars = 50 μm (B) Relative expression of Actin in HUVECs after 5 μM BVT-948 treatment for 24 h. (C) Immunoblotting showing the expression of focal adhesion kinase (FAK) in HUVECs treated with various concentration of BVT-948 for 24 h. (D) Immunoblots showing the levels of phospho-ERK, ERK, and vinculin in HUVECs treated with BVT-948 for 24 h. The membrane was probed with anti-phospho ERK antibody. Then the membrane was reprobed with anti-ERK antibody after stripping.

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